rabbit anti-u2af65 antibody  (ABclonal Biotechnology)

Journal: bioRxiv

Article Title: TDG orchestrates ATF4-dependent gene transcription during retinoic acid-induced cell fate acquisition

doi: 10.1101/2024.04.01.587571

Figure Lengend Snippet: ( A ) Results of MTT assays in wt and TDG-null ECCs treated with increasing concentrations of RA for neurectodermal differentiation or with 1% DMSO for mesodermal differentiation. ( B ) Immunofluorescence detection of the neural marker PAX6 and the cardiomyocyte marker ACTA2 in untreated ECCs (top) and wt (middle) and TDG-null (bottom) cells treated for 72 hours with 1 μM RA. Cells were counterstained with DAPI. ( C ) Phase-contrast microscopy images of wt and TDG- null ECCs treated with 1 μM RA for 6 days. Cells were first grown as embryoid bodies for 2 days and then re-plated in cell-culture dishes for 4 days. ( D ) Quantification of neurite extension from embryoid bodies 4 days after replating. ( E ) RT-qPCR assay of Meis1 mRNA levels in wt and TDG-null ECCs treated with increasing concentrations of RA or 1% DMSO for 48 hours. ( F ) Phase-contrast microscopy images of wt and TDG-null ECCs grown as monolayers and treated with increasing concentrations of RA or with 1% DMSO. White triangles point to regions of RA-induced 3D growth. ( G ) Quantification of 3D growth regions in wt, TDG-null and two clones of Cyp26a1-null ECCs. ( H ) RT-qPCR assay of the pluripotency marker Oct4 , the neural marker Pax6 , and the cardiomyocyte marker Acta2 mRNAs in the indicated cells treated or not with 1 μM RA for 48 hours. ( I ) RT-qPCR assay of Bdnf mRNA in the indicated cells treated or not with 1 μM RA for 48 hours. ( J ) RT-qPCR assay of Tdg mRNA in the indicated cells treated or not with 1 μM RA for 48 hours. ( K ) Western blot detection of TDG in wt, TDG-null, and Cyp26a1-null ECCs. Detection of U2AF65 serves as a loading control. Arrowheads indicate TDG bands and the asterisk indicates a non-specific band. ( L ) RT-qPCR assays for the indicated ATF4/ISR pathway genes in cells treated or not with 1 μM RA for 48 hours.

Article Snippet: Rabbit antibodies against U2AF65 (sc-53942) were purchased from Santa-Cruz Biotechnologies, p70S6K (# 9202) and T389p70S6K-Ph (# 9234) from Cell Signalling Technology.

Techniques: Immunofluorescence, Marker, Microscopy, Cell Culture, Quantitative RT-PCR, Clone Assay, Western Blot, Control

Journal: iScience

Article Title: Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells

doi: 10.1016/j.isci.2022.104289

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-U2AF65 (U2AF2) (1:50) , Proteintech , Cat# 15624-1-AP RRID: AB_2211330.

Techniques: Recombinant, Mass Spectrometry, Sequencing, Modification, Protease Inhibitor, Multiplex sample analysis, Multiplex Assay, Fractionation, Cell Culture, Bicinchoninic Acid Protein Assay, Lysis, Cloning, Gene Expression, SYBR Green Assay, Transfection, Ligation, Microarray, TaqMan Assay, Software, Cell Analysis